Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Hexokinase chemical studies

Hexokinase catalysed syntheses of 6-amino-6-deoxy-D-glucose and -D-gluconate 6-phosphates, 6-amino-6-deoxy-D-fructose 6-phosphate and 1,6-bisphosphate, and 5-amino-5-deoxy-D-ribulose 5-phosphate have been reported. From a study of the properties and reactivity in enzymic reactions of these compounds, it was concluded that they were excellent isosteric analogues of the normal metabolic intermediates except for reactions catalysed by kinases. A preparative enzymic synthesis of uridine 5 -diphospho-2-acetamido-2-deoxy-D-glucose used chemically synthesized 2-acetamido-2-deoxy-D-glucose 1-phosphate and uridine triphosphate as reactants, and a calf liver enzyme extract containing uridine-5 -phospho-A-acetylglucosamine pyrophosphorylase. ... [Pg.117]

Isotopic labeling studies of phosphotranferase reactions culminated in the synthesis of ATP chiral at the y-phosphorus. Chirality was achieved by the synthesis of [y- 0, 0, 0]ATP of one configuration, and the analysis of its chirality was achieved by stereochenucally controlled transfer of the y-phosphoryl moiety to (S)-propane-l,2-diol where the absolute configuration was determined by a chemical/mass spectrometric sequence. The observation of inversion of configuration has been accepted as evidence of an in-line displacement mechanism at phosphorus by the two bound substrates the observation of retention of configuration was used to implicate the existence of a phosphoryl enzyme intermediate in the phosphoryl transfer process. For hexokinase, our case study, the finding is one of inversion, consistent with a direct transfer mechanism. [Pg.27]


See other pages where Hexokinase chemical studies is mentioned: [Pg.349]    [Pg.115]    [Pg.33]    [Pg.384]    [Pg.40]    [Pg.196]   


SEARCH



Chemicals studied

Hexokinase

Hexokinases

© 2024 chempedia.info