Heterologous expression, gene cluster

Heterologous biosynthetic gene cluster expression coupled with comparative... 

Galm, U. and Shen, B. (2006) Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis. Expert Opinion on Drug Discovery, 1, 409. 

Luzhetskyy A, Mayer A, Hoffmann J et al (2007) Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. ChemBioChem 8 599-602... 

Genomic studies disclosed that many of those uncultivable microbes contain the potential to produce new secondary metabolites and there are a variety of routes to access this pool of novel natural products. For example, the community DNA gene clusters can be isolated and the genetic information for secondary metabolism expressed in heterologous hosts.45 47 A more rewarding approach may be to focus on improved cultivation methods.40,48... 

Heterologous expression of a partial gene cluster, followed by the production of biosynthesized unnatural metabolites with altered substituents or a modified core structure (90, 97) (Fig. ID). 

Heterologous expression of a entire gene cluster to make use of the host properties like a fully sequenced genome, the with potential to influence production, improved growth properties, and well developed fermentation... 

Eustaquio AS, Gust B, Galm U, Li S-M, Chater KF, Heide, L (2005) Heterologous Expression of Novobiocin and Clorobiocin Biosynthetic Gene Clusters. Appl. Environ. Microbiol. 71 2452... 

Tang L, Shah S, Chung L, Carney J, Katz L, Khosla C, Julien B. Cloning and heterologous expression of the epothilone gene cluster. Science 2000 287 640-642. 

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