Hemoglobins, stroma-free

A further complication of these reactions is that many nonhemoglobin proteins contain reactive groups and may also be modified to produce new, potentially toxic, contaminants. It has been difficult to produce a pure modified hemoglobin for toxicity studies because most processes start with relatively cmde stroma-free hemoglobin. 

Chan WL, Tang NL, Yim CC, et al. New features of renal lesion induced by stroma free hemoglobin. Toxicol Pathol 2000 28 635. 

Savitsky JP, Doczi J, Black J, et al. A clinical safety trial of stroma-free hemoglobin. Clin Pharmacol Ther 1978 23 73. 

Eieberthal W, Vogel WM,ApsteinCS, Valeri CR Studiesofthe mechanism ofthe vasoconstrictor activity of stroma-free hemoglobin in the isolated perfused rat kidney and rabbit heart. Prog Clin Biol Res 319 407-20,1989... 

Stroma-free hemoglobin preparations have been developed in response to the need for safe, infection-free, sustainable sources of blood in human medicine and to eliminate the need for cross-matching (Table 17.14). Unfortunately, unmodified free hemoglobin has too high an affinity for oxygen, is rapidly eliminated by the kidneys, causes a substantial increase in oncotic pressure and may cause renal injury. For this reason, research has focused on modified polymers of hemoglobin. At the time of writing four products are under development polymerized bovine... 

Samples of blood were obtained from sickle cell anemic individuals who had not been transfused within three months prior to the time of sampling. Stroma-free eoneentrated solutions of human adult hemoglobin were prepared by the method used by Drabkin (5). These solutions were diluted just before use with the... 

Fig. 2. Hemoglobin formed in stroma-free solutions of deoxygenated sickle-cell-anemia hemoglobin. Phase photomicrography, X 375. From John W. Harris, Proc. Soc. Exptl. Biol, and Med. 75, 197 (1950).

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