Hemoglobin basal

For ferrous sulfate, the average efficiency of converting dietary iron into hemoglobin was 52 percent with a coefficient of variation of 19 percent (Table 1). When corrected for the basal dietary ingredients, the average efficiency was 61 percent, with a coefficient of variation of 33 percent. 

However, the hemoglobin response of rats fed a diet that contained diferric phytate was only slightly better than that supported by the low iron basal diet. The response to the tetra-ferric phytate was intermediate between responses to the di- and monoferric phytate. The bioavailabilities of the iron of the three ferric phytates are markedly different. In another experiment (10), a 60-fold excess of phytic acid as sodium phytate did not depress the ability of monoferric phytate to support hemoglobin formation in growing rats. 

The maximum rate of hemoglobin production is assumed to be seven times the normal production. The two constants, K3 and K4, were calculated using the conditions that (1) normal erythropoietin concentration results in normal hemoglobin production and (2) a basal hemoglobin production of. 005 gram/day occurs when erythropoietin concentration is zero. 

The cortex is an amorphous and electron-dense layer composed of at least two sublayers which are abundant in keratin-like proteins and a highly insoluble protein termed cuticulin. The medial layer is a fluid-filled compartment that also contains fine collagenous fibers. In some species, the fluid contains hemoglobin. The composite basal layer contains crosslinked collagen fibers in two to three distinct sublayers which spiral around the nematode at an angle 75° to the longitudinal axis. 

Erythrocyte hemolysis. Hemolysis experiments were performed in PBS using static method, as previously described [2], The degree of hemolysis due to sample activity was calculated as the hemolytic index %H = (Hb - Hbo/Hb,ot) 100%, where Hb is the total amount of released hemoglobin in the assayed sample, Hbo the amount released due to basal hemolysis (200pl erythrocytes incubated with 50pl PBS), and Hb,ot the total amount of released hemoglobin in fully disrupted erythrocytes (0.2 ml erythrocytes in 19.8 ml H2O MilU Q). 

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