Heat Transfer in Stirred Bioreactors

Uptake of oxygen by animal cell cultures is relatively slow. It is therefore anticipated that the associated heat effect will be low. Van t Riet and Tramper (1991) and Nienow (2006) suggest the following equation for estimating the heat released  

Safe engineering practice requires design for the worst conditions. Therefore, the OUR in Equation 7B.19 to be used is the highest value/peak OUR for animal cells listed in Table 7B.6 5 mmol/l/h. Based on this maximum OUR, the heat effect is 

Cooney et al. (1969), Junker (2004), and Benz (2011) suggest that the heat transfer duty can be estimated as 110 kcal/mol of oxygen uptake. Using this value, the heat evolved is 550 kcal/mVh, which is 25% lower than the value obtained from 

STIRRED TANK REACTORS EOR CELL CULTURE TECHNOLOGY 

FIGURE 7B.20 Bioreactor with cooling jacket. (Reproduced from Benz (2011) with permission from American Institute of Chemical Engineers. 2011, American Institute of Chemical Engineers (AIChE).) 

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