Heat-Assisted Polymerase Chain Reaction

MICROWAVE HEAT-ASSISTED POLYMERASE CHAIN REACTION 

The microdissected section fragments in the TE buffer are transferred from the slide to a 0.5-ml plastic tube and centrifuged at 12,000 g for 15 min. The supernatant is discarded, and the tissue pellet is resuspended in 20 xl of mineral oil and heated in a microwave oven at 800 W for 7 min. After resuspension in an aliquot of TE buffer, the sample is added to the master mix for PCR amplification. The details of amplification and sequence analysis are presented by Alcock et al. (1999). 

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MICROWAVE HEAT-ASSISTED POLYMERASE CHAIN REACTION... 

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