Harmalae semen

Harmalae semen Powdered drug (Ig) is extracted with 10 ml methanol for 30 min under reflux, The filtrate is diluted 1 10 with methanol and 20 d i.s used for TLC. Strychni semen Powdered seeds (1 g) are defatted with 20 m n-hexane for 30min under reflux. The defatted seeds are then extracted with 10ml methanol for lOmin under reflux. A total of 30pl of the filtrate is used for TLC. 

Fig. 9 Harmalae semen Syrian (wild) rue Peganum harmala L. Zygopbyllaceae...

Drug sample 1 Harmalae semen, (methanol extract, 30 il)... 

Fig. 9A Harmalae semen. The carbolin derivatives harmalol (Tl), harmaline (T2) and harmine (T4) are found as bright blue fluorescent zones in solvent A in the R, range 0.1-0.75. The Harmalae semen sample 1 shows as major alkaloids harmalol and harmaline in the low Rf range 0.05-0.25 and harmine in the upper Rf range 0.75.

---