Hansenula anomala, flavocytochrome

Hammett s-constants, of astatophenols, 31 66 Hansenula anomala, flavocytochrome bj from, 36 260-261... 

Equations (11.10) and (11.11) do not yield exactly the same value for protein with a molecular mass of 235 kDa, such as flavocytochrome b2extracted from the yeast Hansenula anomala, the rotational correlation times calculated from Equations (11.10) and (11.11) are 97 and 90 ns, respectively. 

Flavocytochromes 2 2-hydroxyacid dehydrogenases found in the inter-membrane space of yeast mitochondria where they couple oxidation of the substrate to reduction of cytochrome c. Examples include the enzymes from Saccharomyces cerevisiae and Hansenula anomala, both of which are l-lactate dehydrogenases (Chapman et al., 1998), and the enzyme from Rhodotorula graminis which is a L-mandelate dehydrogenase (Ilias et al., 1998). This article will concentrate on the flavocytochrome 2 (L-lactate cytochrome c oxidoreductase) from S. cerevisiae (Bakersi yeast), since this is by far the most studied of these enzymes (Chapman et al., 1991). Therefore, throughout this article, the term flavocytochrome 2 will refer specifically to the enzyme from S. cerevisiae unless otherwise stated. 

Fig. 4. Sequence alignment of flavocytochromes 62 and glycollate oxidase. The amino acid sequences of the mature forms of flavocytochromes 62 from Saccharomyces cerevisiae (Scb2) and Hansenula anomala (Hab2) and of glycollate oxidase from spinach (SpGO) are shown along with a consensus (Con) wherein all three sequences are identical. The interdomain hinge region and the proteinase-sensitive loop of flavocytochromes are boxed.

Thomas, M. A., Gervais, M., Favaudon, V. and Valat, P. 1983, Study of the Hansenula anomala yeast flavocytochrome-b2-cytochrome-c complex 2. Localization of the main association area. European Journal of Biochemistry 135, 577-581. 

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See also in sourсe #XX -- [ , ]

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