Hairless mouse skin, effect ethanol

Effects of Ethanol on the Transport of /3-Estradiol in Hairless Mouse Skin... 

This paper describes the striking effects of ethanol on the transport of 3-estradiol in hairless mouse skin. 

The purpose of this report is to present results on (a) the effect of ethanol on the transport of 8-estradiol across hairless mouse skin and (b) the effect upon the effective permeability coefficient as solvent compositions are independently varied in the donor and receiver chambers. Also, since there is evidence for pore formation, at least at the highest ethanol levels, a novel pore model... 

Ghanem, A.-H. et al. The effects of ethanol on the transport of B-estradiol and other permeants in hairless mouse skin II. A new quantitative approach. Journal of Controlled Release 6 75-83, 1987. 

Liu P, Higuchi WI, Song W, et al. Quantitative evaluation of ethanol effects on diffusion and metabolism of P-estradiol in hairless mouse skin. Pharm Res 1991 8(7) 865-872. 

Behl et al. studied the effect of prolonged contact of hairless mouse skin with water on permeability coefficients. The authors showed that permeability coefficients increase after extended periods of hydration. Because other permeability coefficients in the database we have assembled were measured on previously unhydrated skins or skins that were hydrated for short periods, the permeability coefficients with the shortest hydration time (0.3 to 0.8 h) from Table 1 were selected for the validated database. Permeability coefficients were determined with either water or ethanol as a copenetrant. The concentrations were dilute (alcohol concentrations less than 10 M) and probably were not damaging. Six reported measurements were averaged for methanol, two for ethanol, and two for butanol, and permeability coefficients were reported singly for hexanol, heptanol, and octanol. Although this article did not specify the diffusion cell temperature, subsequent articles by the same authors describing similar data indicated that the temperature was 37°C (e.g., Behl and Barrett, 1981 Behl, El-Sayed, et al., 1983 Behl, Linn, et al., 1983). It seems hkely that the temperature was also 37°C in the experiments described in this article. 

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