Hair analysis chromatographic procedures

Hair analysis by chromatographic procedures was reviewed in 1992 by Moeller. Analytical methods for the detection of drugs in hair were reviewed for amphet-amines, °7 cannabinoids, cocaine, ° and opiates. "... 

Analytical procedures involving reduction and determination of mercaptan are not accurate determinations of cystine in permanent-waved hair or in hair treated with mercaptan because mixed disulfide is reduced to mercaptan during analysis adsorbed mercaptan can also interfere in the determination. Procedures that do not involve reduction of hair such as ninhydrin detection (alpha-amino group) or dinitrofluorobenzene (DNFB) reaction followed by chromatographic separation [1, 58] discriminate between mercaptans and, therefore, should be better analytical procedures for detecting the different types of mercaptans and disulfides actually present in permanent-waved hair. 

Matrix compounds in hair dyeing consist of surfactants, emulsifiers, preservatives, conditioning agents, and additives for pH control/adjustment. They are essentially noncolor additives that are functional agents in hair dyes see Table 6-4. Vincent et al. [35] describe a procedure for the extraction of matrix compounds from oxidation hair dyes to allow for the clean chromatographic separation of the hair dye compounds for analysis. 

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