Gutless Ad vectors

The development of the gutless Ad vector, which is deprived of nearly all viral sequences, prevents elimination of transduced cells by the immune system, thus allowing long term transgene expression. However, the vector DNA genome exists episomally and is nomeplicating. Therefore, transgene expression may be lost over... 

One of the major concerns in the use of Ad vectors for gene therapy purposes is the induction of an innate immune response. Administration of an Ad vector causes inflammation of the infected tissue, especially in the liver (110, 113-115). The induction of chemokine production at the infected site attracts neutrophils, leading to necrosis and apoptosis in the liver. The development of gutless Ad vectors markedly reduce inflammatory responses and cellular infiltration (116). However, inflammation induced by the viral capsid proteins itself cannot be prevented (110). 

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