Guanidinium isothiocyanate

Total RNA can be prepared using commercially available kits according to the protocols of the manufacturer or by the guanidinium isothiocyanate method, followed by caesium chloride centrifugation (CsCl). Details are published in handbooks of molecular biology techniques. In our hands, both methods are equivalent, but if budgets are limited, the CsCl method may be more suitable for the preparation of large amounts of RNA. 

A bis(isothiocyanate) was treated with a diamine in the ring-closure step. The resulting cyclic bis(thiourea) was converted into the bis(guanidinium)-containing macrocycle. 

AI3-18430 EINECS 209-812-1 Guanidine, monothiocyanate Guanidine thiocyanate Guanidinium thiocyanate Isothiocyanic acid, compd. with guanidine (T.1) NSC 2119 Thiocyanic acid, compd. with guanidine (1 1) USAF EK-705. Potent protein denaturant used in isolation of intact DNA, RNA. Crystals mp= 117°. Dajac Labs. Eastman Chem. Co. Ftuka U.S. BioChem. 

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