Growth reactions energy input

Temperature In case of a reaction controlled process, changes of reaction temperature would influence the rate of the process [34]. However, experiments with a reagent inflow rate of 0.3 mol/h and a specific energy input of 0.16 W/kg showed no distinct influence of temperature for either of the two solution types tested (see Figure 4 (c)). Based on the evidence presented above the crystal growth of HH is concluded to be a diffusion controlled linear process under the investigated conditions. A more detailed discussion can be found in a previous publication [28]. 

Determine if the CO shift reaction can serve as an energy-yielding step in darkness. If verified, this energy can be used to sustain cell growth and to induce new CO shift enzymes to support H2 production for a longer duration without the need for light input. 

In Nature s genomes, repheation fidelity is primarily a kinetic function of the mechanistically complex DNA polymerases [43]. These enzymes employ activated phosphoric acid anhydrides leading to a final product-template duplex. The third step, dissociation of the product strand from the template, requires an additional input of chemical energy and a separate set of protein catalysts. Therefore each step in DNA replication requires a critical and complex set of translation products to ensure the production of a single copy of the template, and for autonomous growth, the template must be of sufficient size to encode all of the required catalysts for the process. The notion that these templates must encode catalysts, or possibly even the templates themselves serve as catalysts for more than one reaction, becomes an important feature for the development of template autonomy. 

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