Grafted Anion-Exchange Resins

Ion-exchange chromatography is widely used in the analysis of protein variants that may include glycosylated [309], phosphorylated [310], deamidated [311], truncated [312], and oxidized [313] forms. Chromatographic separations of 

Polymer-based tentacular materials for anion-exchange chromatography of proteins are offered under the trade name Thermo Scientific Dionex ProPac. The specifications of the two different ProPac anion exchangers currently available are summarized in Table 3.37. 

Tentacular anion exchangers are predominantly used for the following separation problems  

Base matrix material Ethylvinylbenzene 55% cross-linked with divinylbenzene Ethylvinylbenzene 55% cross-linked with divinylbenzene 

Functional group Tertiary amine Quaternary amine 

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Hydroxide-Selective Grafted Polymers Dionex (now part of Thermo Fisher Scientific) also introduced a hydroxide-selective, surface-functionalized EVB/DVB resin featuring a covalently attached anion-exchange polymer under the trade... 

Imoto R, Kosaka Y, Shimizu A (1966) Process for manufacturing anion-exchange membranes from a graft copoloymer of SBR and a vinylpyridine reacted with an epoxy resin. US Patent 3258435... 

Guerra and Palla ° have recently prepared some unique graft copolymers by reacting polystyryl anions (living polymer) with EMA copolymer. This general reaction is being explored to produce new cation-exchange resins. 

Other milestones are grafting by radical, cationic, anionic and coordinative polymerization use of semisynthetic and synthetic polymers as ion exchange resins " and catalysts which, in some cases, mimic and even surpass the efficiency of enzymes and solid phase peptide synthesis by Merrifield and Letsinger. ... 

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