Gradients generated at atmospheric pressure

The gradient systems in which the solvents are mixed at atmospheric pressure are generally inexpensive compared with high-pressure systems. The mixture is 

Since gradient elution was first used in chromatography, many more or less complex devices have been used. We shall describe first devices for mixing two 

Probably the simplest means of making a gradient is periodically to change the solvent reservoir manually. However, in this instance only step gradients can be formed. 

Three different types of atmospheric pressure, two-solvent gradient generators 

In the simplest form, two vessels are connected by a stop-cock as shown in Fig. 1. The mixing vessel is first filled with the solvent of low eluting power, 

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