Gold nanoparticles light scattering

Raschke, G., Kowarik, S., Franzl, T., Sonnichsen, C., Klar, T. A., Feldmann, J., Nichtl, A., Kurzinger, K. (2003). Biomolecular recognition based on single gold nanoparticle light scattering. Nano Lett 3, 935-938. 

Raschke G, Kowarik S, Franzl T et al (2003) Biomolecular recognition based on single gold nanoparticle light scattering. Nano Letters 3 935... 

Liu, X., Dai, Q., Austin, L., Courts, J., Knowles, G., Zou, J. H., Chen, H., and Huo, Q. (2008). A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering. J Am. Chem. Soc. 130 2780-2782. 

Figure 20.3 Enhanced optical properties of gold nanoparticles resulting from the interaction of light with a gold nanoparticle including absorption, Mie scattering, fluorescence and surface nhanced Raman scattering of adsorbed molecules. Reprinted with permission from ref 3. Copyright 2007 Future Medicine.

The nanoparticles can be incorporated into cells by incubation the nanoparticies suspended in DMEM medium with the cells for 12 to 48 hours. From dark field light scattering imaging [54], it has been established that gold nanoparticies are taken intracellulariy into these cell lines. The nanoparticies are accumulated inside cytoplasm of live cells by receptor-mediated endocytosis processes. Figure 20.16 shows that for most of samples gold nanospheres quench the protein fluorescoice at 12 h incubation time. However, in some samples fluorescence enhancement is observed. 

Figure 28.11 Light scattering spectra and images of single gold nanoparticles dispersed on/in a single living NIH3T3 cell.

Since light scattering spectra of gold nanoparticles reflect the environmental condition, simultaneous monitoring of the position and spectrum give us a new way to understand dynamic aspects of single living cells. When they are dissolved in... 

Itoh, T., Asahi, T. and Masuhara, H. (2001) Femtosecond light scattering spectroscopy of single gold nanoparticles. Appl. Phys. Lett., 79, 1667-1669. 

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