Goat anti-mouse reagent

Fluorescent immunoassay was also conducted. For instance, physisorption of goat anti-mouse IgG (biotin-conjugated) was first made to the PDMS surface for the immunoassay of IgG. Then neutravidin was introduced to bind to the biotin on the surface. Immobilization of biotinylated goat anti-human IgG on the neu-travidin-coated channel was first achieved by confining the reagent flow at a T-intersection on the PDMS chip. The antigen (Cy5-human IgG) was applied via a perpendicular channel. Fluorescence measurement allowed the... 

Control line reagent The goat anti-mouse IgG is isolated by protein A column as above from sera of goats immunized with mouse IgG and stored at -20°C in 1 mL aliquots. 

Dilute the BSA-CL and goat anti-mouse IgG to lmg/mL in PBS (pH7.2) for application as the test and control line capture reagents, respectively. 

Suitable secondary antibody reagent, e.g., phycoeiythrin-conjugated Goat F(ab )2 anti-Mouse antibody (R D Systems). 

---