GNAT structure

The structures of four HATs (yeast Hatl, Gcn5 from yeast (y) and Tetrahymena thermophUa (t), human PCAF, yeast Hpa2) from the GNAT family have been solved so far [15-19]. These structures show that the evolutionarily conserved sequences of these proteins - termed motifs A, B, C, D [14] - form a conserved structural core. This core domain is constituted by a central highly curved five-stranded... 

Structure of a HAT from the p300/CBP family has been long awaited, since these enzymes have no sequence similarity with enzymes of the GNAT and MYST families. The structure of the p300 HAT domain was solved very recently in complex with a Lys-GoA bi-substrate inhibitor, which required a highly intricate expression and purification protocol in order to produce a protein amenable to crystallization studies [24]. Analysis of the structure revealed however that, despite no sequence conservation, a similar structural core is observed as for the GNAT and MYST... 

Methoprene, a juvenile hormone analog, is structurally similar to insect juvenile hormones (Figure 4.11) such as juvenile hormone III. It is used for control of mosquitoes (floodwater mosquitoes, 2-4 instars), cigarette beetles, horn flies, fungus gnats (on mushroom), fleas, etc. The oral LD50 for methoprene in rats is >34,600 mg/kg. 

S, Roderick SL, Blanchard JS. Structure and functions of the 45. GNAT superfamily of acetyl transferases. Arch. Biochem. Biophys. 2005 433 212-226. 

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