Glycyl-leucyl-phenylalanine

E. Abderhalden und P. Rona. Das Verhalten von Leucyl-phenylalanin, Leucyl-glycyl-glycin und von Alanyl-glycyl-glycin gegen Presssaft der Leber vom Rinde. Zeit. physiol. Chem., igo6, 49, 31-40. 

Figure 7.11 Chromatograms of a mixture of amino acids and peptides eluted with 0.02 M SDS mobile phases containing (a) 3% 2-propanol, (b) 12.5% acetonitrile, and (c) 3% tetrahydrofuran. Compounds (1) tyrosine, (2) methionine, (3) alanyi-tyrosine, (4) tryptophan, (5) aspartyl-phenylalanine, (6) leucyl-tyrosine, and (7) glycyl-leucyl-tyrosine. Reprinted from Ref 10 with permission of Elsevier.

Phenylalanine reacts with ninhydrin in the presence of a dipeptide (usually glycyl-L-leucine or L-leucyl-L-alanine) to form a fluorescent product. The fluorescence is enhanced and stabilized by the addition of an alkaline copper reagent to adjust the pH to 5.8 and the resulting fluorescence is measured at 515 nm after excitation at 365 nm see Procedure 10.2. 

Glycyl glutamic acid Glycyl proline Glycyl tryptophan Leucyl glycyl phenylalanine Tryptophanyl alanine Tryptophanyl glycine Tryptophanyl tryptophan Tryptophanyl tyrosine... 

Phenylalanine pH 2.0 Phenylalanine pH 12.0 Tyrosine pH 6.0 Tryptophan pH 2.0 Tryptophan pH12.0 Tyrosyl glycine Glycyl tyrosine Leucyl tyrosine Tyrosyl tyrosine Tyrosine anhydride Tryptophan ethyl ester Glycyl tryptophan pH 2,0... 

Those not Hydrolysed. Aminobutyryl-aminobutyric acid B. Valyl-glycine. Glycyl-phenylalanine. Leucyl-proline. 

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