Glycosylation receptors

G. Harms, C. D. Dijkstra, Y. C. Lee, and M. J. Hardonk, Glycosyl receptors in macrophage subpopulations of rat spleen and lymph node. A comparative study using neoglycoproteins and monoclonal antibodies EDI, ED2 and ED3, Cell Tissue Res. 262 35-40 (1990). 

Supports prepared with sialic acid have also been used in the purification of insulin, as this sugar and N-acetylglucosamine have been found to take part in the interaction between insulin and its glycosylated receptor. The affinity between insulin and sialic acid-bearing supports has been found rather strong i a ... 

One glycosylation site exists on the P suburhts of human LH [53664-53-2 and TSH [64365-92-OJ ie, Asn-30 (Fig. 4). In some species, Asn-13 of LH-P is glycosylated (48). FSH-P suburnt [58857-12-8] is glycosylated at two sites, Asn-13 and 30. Based on interactions of synthetic peptides with the LH receptor, loops formed by P93—100 and P38—57 may be essential for hormone bioactivity (48). Highly conserved sequences between residues 31—37 have been implicated in the formation of the a—P suburnt dimer (48), which is absolutely necessary for the expression of bioactivity. 

Fig. 5. Schematic diagram of the presumed arrangement of the amino acid sequence for the 5-opioid receptor, showing seven putative transmembrane segments three intracellular loops, A three extracellular loops, B the extracellular N-terrninus and the intracellular C-terrninus, where (0) represents amino acid residues common to ] -, 5-, and K-receptors ( ), amino acid residues common to all three opioid receptors and other neuropeptide receptors and (O), other amino acids. Branches on the N-terruinal region indicate possible glycosylation sites, whereas P symbols in the C-terminal region indicate...

Fig. 12. Model for the P-adenergic receptor. It is proposed that the receptor possesses seven hydrophobic heUces that span the plasma membrane and are connected by alternating extracellular and intracellular loops (79). The site of glycosylation is represented as CHO. Reprinted with permission from Elsevier...

Fraser, N. J., Wise, A., Brown, J., McLatchie, L. M., Main, M. J., and Foord, S. M. (1999). The amino terminus of receptor activity modifying proteins is a critical determinant of glycosylation state and ligand binding of calcitonin-like receptor. Mol. Pharmacol. 55 1054-1059. 

Interleukin 2 (IL-2) is a 15.5 kDa glycosylated protein produced by helper T-cells in response to an antigen and interleukin-1. Ligation of the IL-2 receptor (expressed by a number of lymphocytes) by IL-2 stimulates growth, differentiation and survival. 

Arrhythmogenic Right Ventricular Dysplasia/ Cardiomyopathy Aiteriogenesis Arteriosclerosis Arylhydrocarbon Receptor L-Ascorbic Acid ASF Family of Transporters Asn-linked Glycosylation ASON... 

The disorders listed are discussed further in other chapters. The table lists examples of mutations affecting receptors, a transporter, an ion channel, an enzyme, and a structural protein. Examples of altered or defective glycosylation of glycoproteins are also presented. Most of the conditions listed affect the plasma membrane. 

It was concluded that, as a minimum condition, the receptor has sites that respond to hydroxyl, alkoxyl, and the glycosyl (or adjacent) parts of the molecule (R) (see Fig. 23). The orientation of the B-ring is critical. It must be in quasiaxial orientation for active interaction with the receptor. ... 

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