Glycosphingolipids neutrophil

Karlsson, A., Miller-Podraza, H., Johansson, P., Karlsson, K. A., Dahlgren, C., and Teneberg, S. (2001). Different glycosphingolipid composition in human neutrophil sub-cellular compartments. Glycoconj. J. 18, 231-243. 

Structural Studies of Neutral Glycosphingolipids of Human Neutrophils by Electron Impact/Desorption Mass Spectrometry... 

Isolation of the neutra-L glycosphingolipids. In a typical extraction procedure, 10purified human neutrophils yielded 100-150 mg of total glycosphingolipids. As shown in Table I, glycosphingolipids account for approximately 10% of the total cellular dry weight of the neutrophil. Separation of the total neutrophil lipids by DEAE-sephadex and silicic acid column chromatography yielded 70-100 mg of neutral glycosphingolipids from lO cells. 

Figure 1. Thin-layer chromatography of fractions 1-lV isolated from human neutrophils. The separation is on a plate of silica gel 60 (HPTLC) in solvent system A. S erythrocyte glycosphingolipid standards 1-4 human neutrophilglycosphingo-...

The studies discussed above have all dealt with glycosphingolipids isolated from neutrophils that were derived from normal whole blood. Wherrett (16) has presented detailed structural analysis of a tetraglycosylceramide isolated from polymorphonuclear leukocytes obtained from the urine of a patient with a urinary tract infection. This glycosphingolipid was determined to be lactoneotetraosylceramide. 

The data presented in this report allow the assignment of partial structures for four neutral glycosphingolipids of human neutrophils Hexose-O-Cer, Hexose-Q-Hexose-O-Cer, Hexosamine -0-Hexose-0-Hexose-0-Cer and Hexose-O-Hexosamine-O-Hexose-0-Hexose-0-Cer. 

In addition to these structures, human neutrophils also contain eight to twelve gangliosides and a few species of glycosphingolipids with more than four saccharide units. Purification and structural analyses are currently underway in our laboratory. 

Fig. 2 Binding of 123I-labeled neutrophil-activating protein from Helicobacter pylori (HP-NAP) to derivatized NeuGca3neolactohexaosylceramide (NeuGca3Gaip4GlcNAcp3Gaip4GlcNAc P3Gaip4GlcpiCer) on thin-layer chromatograms. Glycosphingolipids detected with anisaldehyde...

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