Glycosphingolipids metabolism studies

With the aim of studying the glycosphingolipid metabolism, thiolactosylcera-mide (39c) (Scheme 12) was obtained. The corresponding free disaccharide has been shown to be totally resistant to the action of a mixture of GMi- -galacto-sidase and glucocerebrosidase [38]. 

More recently, we have been studying glycosphingolipid metabolism in single AtT20 cells. Glycosphingolipids are among the most common molecules on neural cell surfaces. These... 

Essaka, D. C. Prendergast, J. Keithley, R. B. Palcic, M. M. Hindsgaul, O. Schnaar, R. L. Dovichi, N. J. Metabolic cytometry Capillary electrophoresis with two-color fluorescence detection for the simultaneous study of two glycosphingolipid metabolic pathways in single primary neurons. Anal. Chem. 2012, 84, 2799-2804. 

Thus, the analytical HPLC method for glycolipids is proving useful for a variety of studies related to glycosphingolipid function and metabolism. 

The glycosphingolipids and their complicated metabolism described above have all been studied in vertebrates and, in most cases, are almost entirely known from a few mammalian species. Very few animals more primitive than fish have been examined in detail for their content of glycolipids. Some recent analyses of Hori, Itasaka, Sugita and their colleagues illustrate the startling results that can appear from the invertebrates and suggest possible new pathways of glycosphingolipid assembly. 

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