Glycosidases mutant

Glycosidase mutants (thioglycoligase and thioglycosynthase) in the synthesis of S-linked glycosides... 

M. Jahn, H. Chen, J. Mullegger, J. Maries, R. A. Warren, and S. G. Withers, Thioglycosynthases Double mutant glycosidases that serve as scaffolds for thioglycoside synthesis, Chem. Commun. (Camb.) (2004) 274—275. 

Besides (thio)glycosynthases and thioglycoligases, several other mutant glycosidases were produced with abolished hydrolytic activity in favour of synthesis transsialidase from Trypanosoma rangeli37 or p-transglycosidase from Thermits thermophilus.153 A new p-fucosidase activity was introduced into E. coli p-galactosidase by genetic manipulation.37... 

Thioglycosynthase a double mutant of a retaining glycosidase, in which both the catalytic nucleophile and the catalytic acid-base residue at the active site have been substituted by non-nucleophilic residues. It... 

Figure 2. Azide rescue of an acid/base catalyst (A) and a nucleophile (B) mutant of a retaining b-glycosidase. R aglycon group.

Moracci, M., Trincone, A., Perugino, G., Ciaramella, M. and Rossi, M. (1998) Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus dependence of the mechanism on the action of external nucleophiles. Biochemistry, 37-17262 17270. 

Mackenzie LE, Wang QP, Warren RAJ, Withers SG. Glycosyn-thases Mutant glycosidases for oligosaccharide synthesis. J. Am. Chem. Soc. 1998 120 5583-5584. 

Jahn M, Maries J, Warren RA, Withers SG. Thioglycoligases mutant glycosidases for thioglycoside synthesis. Angew. Chem. Int. Ed. Engl. 2003 42 352-354. 

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