Glycosaminoglycans polysaccharide lyases

Polysaccharide lyase Family 8 (PL 8). This family encompasses lyases acting upon mammalian connective tissue glycosaminoglycans, and for this reason is the best investigated, with a number of crystal structures available. It has a two-domain fold with an (a/ot)5 toroid connected to a domain almost exclusively of (3-sheet. The reactions catalysed are syn eliminations, with available evidence indicating an irreversible 1cb mechanism, in which the intermediate enolate is stabilised by hydrogen bonding, rather than metal ions, and both deprotonation of C5 and protonation of 04 are carried out by the same tyrosine residue. 

Table 2. Properties of polysaccharide lyases acting on glycosaminoglycans...

Polysaccharide lyases can be combined with separation methods such as chromatography and electrophoresis for the preparation of glycosaminoglycan oligosaccharides for biological evaluations as well as for disaccharide analysis, oligosaccharide mapping and polysaccharide sequencing. 

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