Structural analysis glycoprotein

V. Structural Analysis of Glycopeptides and Glycoproteins Containing Unusual... 

Recent Developments in the Use of 13C-N.m.r. Spectroscopy for the Structural Analysis of Oligosaccharides of Glycoproteins... 

The analysis of the anomeric-carbon region of these two glycoproteins provided additional information not available by more conventional methods. Any structural analysis of such a complex system must be based on the assignments of the various signals in the spectra. In Table III are tabulated the assignments of the anomeric-carbon regions in Fig. 

Vliegenthart, J.F.G., Dorland, L., and van Halbeek, H. (1983) High-resolution, lH-nuclear magnetic resonance spectroscopy as a tool in the structural analysis of carbohydrates related to glycoproteins. Adv. Carbohydr. Chem. Biochem. 41, 209-374. 

METHYLATION TECHNIQUES IN THE STRUCTURAL ANALYSIS OF GLYCOPROTEINS AND GLYCOLIPIDS... 

Multiple-ion monitoring is, however, of considerable value in structural studies, but only if model compounds of known structure are available for comparison. Such an approach has been used in the study of the carbohydrate structures of glycoproteins from different tissues.50 Separation of glycopeptides obtained from various tissues was performed on columns of concanavalin A-Sepharose. Structural analysis by multiple-ion monitoring of partially methylated, alditol acetates derived from the various fractions indicated that the glycopeptides were separated according to the linkage pattern of mannose (see Fig. 1). 

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