Platelets membrane, glycoproteins from

Human-platelet membranes have been extracted with lithium 3,5-diiodosalicylate, and the soluble glycoproteins separated on O-phosphonocellulose.849 The purified, membrane glycoprotein, molecular weight 100,000, was immunochemically identical to a sample purified from platelet-membrane extract by means of con A-Sepharose affinity chromatography. No further characterization was reported,... 

Painter RG, PRODOUZ KN, GAARDE W. isolation of a sul x ulation of glycoprotein llbllla from platelet membranes that is bound to memlx ane actia JCdl Biol 100 652-657,1985. 

CD31 JC/70A 100-kD glycoprotein in endothelial cells and 1 30 kD glycoprotein in platelets Membrane preparation of spleen from patient with hairy cell leukemia Cell Marque NA HIER... 

The two platelet membrane glycoproteins for which the strongest evidence exists at the present time that they are thrombin receptors are a specific form of the GPIb-DC-V conq>lex and PARI, the protease-activated thrombin receptor these will be discussed next in this review. Based on their apparent ability to form complexes with a hrombin, several other proteins had previously t en proposed as thrombin receptors but in only a few cases were the necessary further studies carried out to test these hypotheses. Protease nexin 1 was proposed as a thrombin receptor based on the similar time courses of complex formation and platelet activation but it was subsequently found that protease nexin 1 cannot be a receptor since it is an internal conq>onent of platelets that is expressed on the surface only ater activation. Glycoprotein V has also been proposed as a thrombin receptor based on the feet that it can be cleaved by low concentrations of a-thrombin but subsequent studies (reviewed in ) showed that there was no consistent relationship between the rate or extent of GPV hydrolysis and the extent of platelet activation induced by a-thrombin. Another thrombin-activatable receptor, PAW, has been identified in mouse platelets but not in human platelets using a reverse transcriptase/ polymerase chain reaction approach (unpublished data and S.Coughlin, personal communication). PAR3 has, however, been reported cloned from a human platelet cDNA library. ... 

Takahashi H, Hanano M, Moroi M, Shibata A Platelet membrane glycoprotein VI (GPVI) is necessary for collagen-induced aggregation and adhesion and anti GPVI antibody induces platelet aggregation An evidence obtained from a patient with systemic lupus erythematosus. Thromb Haonost 73 1197, 1995 (abstract)... 

A complex glycoprotein I, consisting of two polypeptides (mol. wt. 2.1 x 10 and 1.5 X 10 ) has been isolated from human platelet membranes. Glyco-... 

Purification of the peptidase active against insulin B-chain, etc., from rat kidneys Separation of enzyme cofactors Afllnity chromatography of anti-(lacto-JV-difucohexaose I) antibody immunoglobulin Purification of membrane glycoproteins from human-blood platelets... 

Jenkins, C.S., Phillips, D.R., Clemetson, K.J., Meyer, D., Larrieu, M.J., and Luscher, E.F. 1976. Platelet membrane glycoproteins implicated in ristocetin-induced aggregation. Studies of the proteins on platelets from patients with Bemard-Soulier syndrome and von Willebrand s disease. J.Clin.Invest 57 112-124. 

The platelet surface contains aggregin, a membrane protein with a molecular weight of 100 kDa, which has physical and immunochemical properties that differ from those of platelet glycoprotein Ilia. 

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