Glycoprotein sequence, mapping

Khoo, K.-H., Chatterjee, D., Caulfield, J.P., Morris, H.R. and Dell, A. (1997b) Structural mapping of the glycans from the egg glycoproteins of Schistosoma mansoni and Schistosoma japonicum. Identification of novel core structures and terminal sequences. Glycobiology 7, 663-677. 

Hyaluronidases are generally glycoproteins. This has been confirmed for testicular [67], Staphylococcus aureus [68], lysosomal [59], lizard venom [21], and stonefish venom [22] hyaluronidases. The amino acid compositions of testicular [67], Staphylococcus aureus [68], lizard venom [21], and stone-fish venom [22] hyaluronidases have been determined. Of the latter type, the N-terminal sequence has been derived. The hyaluronidase gene of Streptococcus pyogenes bacteriophage H4489A has been expressed in Escherichia coli and the full nucleotide sequence has been derived. This made possible a mapping of the full amino acid sequence of the enzyme [69]. 

Y. Mechref, M. V. Novotny. Mass spectrometric mapping and sequencing of N-linked oligosaccharides derived from submicrogram amounts of glycoproteins. Anal. Chem. 1998, 70,455-463. 

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