Glycopeptides peptide composition

A characteristic but unexplained feature of the peptide composition of all of these glycopeptides and peptido-oligosaccharides (see Table V) is the absence, or presence in traces only, of the aromatic amino acids. The presence of hydroxy-L-proline in some of these fractions might indicate that the glycopeptide components of these fractions are derived from connective-tissue components, as this... 

Carbohydrate and Peptide Composition of Urinary Glycopeptides and Peptido-oligosaccharides... 

For most free amino acids and small peptides, a mixture of alcohol with water is a typical mobile phase composition in the reversed-phase mode for glycopeptide CSPs. For some bifunctional amino acids and most other compounds, however, aqueous buffer is usually necessary to enhance resolution. The types of buffers dictate the retention, efficiency and - to a lesser effect - selectivity of analytes. Tri-ethylammonium acetate and ammonium nitrate are the most effective buffer systems, while sodium citrate is also effective for the separation of profens on vancomycin CSP, and ammonium acetate is the most appropriate for LC/MS applications. 

Table III. Molar Composition of Core Protein (Peptide) of BSM, Trypsin and /3-Elim. Glycopeptides (Pro = 3)...

Glycopeptides. Among the vast class of peptide antibiotics, the only ones commonly used are members of this family, denoted as dalbaheptides in view of their mechanism of action (d-aknine-D-alanine Mnding) and their composition (heptapeptides). The most well known are vancomycin and teicoplanin, produced by Amycolatopsis orientalis and Actinoplanes teichomyceticus, respectively (O Fig. 9.6). 

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