Glycopeptide complex separation

Figure 6a. Analytical separations of a crude glycopeptide complex (isocratic). Sample crude antibiotic extract. Column Beckman Ultrasphere ODS, 5 jzm, 4.6 x 150 mm. Mobile phase 35%, acetonitrile in 0.1 M KH2P04pH 3.2. Flow 1.5 ml/min. Detection ...

Figure 7. Small-scale preparative separation of glycopeptide complex. Sample crude antibiotic extract (2 g). Column ...

Figure 9a. Small-scale preparative separation of glycopeptide complex on Magnum 40 column (4.5 x 50 cm). Sample purified antibiotic (2 g). Column Whatman Partisil Prep 40 0DS-3 (37-60 jxm). Mobile phase 20 to 26% acetonitrile in 0.1 M KH2PO4PH 6.0. Flow 100 ml/min. Detection 210 nm.

Figure 9b. Recovery data for small-scale separation of glycopeptide complex on Magnum 40 column. Sample purified component I. Column Beckman Ultrasphere 0DS, 5 xm, 4.6 x 150 mm).

Figure 10a. Scale-up preparative separation of glycopeptide complex (25 g run). Sample crude antibiotic complex (25 g).

Analytical Properties Substrate has 38 chiral centers and 7 aromatic rings surrounding 4 cavities (A, B, C, D), making this the most structurally complex of the macrocyclic glycopeptides substrate has a relative molecular mass of 2066 this phase can be used in normal, reverse, and polar organic phase separations selective for anionic chiral species with polar organic mobile phases, it can be used for a-hydroxy acids, profens, and N-blocked amino acids in normal phase mode, it can be used for imides, hydantoins, and N-blocked amino acids in reverse phase, it can be used for a-hydroxy and halogenated acids, substituted aliphatic acids, profens, N-blocked amino acids, hydantoins, and peptides Reference 47, 48... 

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