Glycolipid labeled neutral

Labelled Neutral Glycolipids. Neutral glycolipids were labelled with -galactose. As was seen with the ganglioside, the butyrate affected the neutral glycolipid patterns but the most marked alterations appeared to be due to changes in the lipid moeities. 

Thin Layer Chromotography. Unless otherwise stated, all thin layer chromotography was on plates coated with silica Gel G (E. Merck, Dramstadt). All the solvents were mixed on a volume basis. Neutral glycolipid fractions were developed in chloroform methanol water (60 35 6.5). Labelled fucolipid fractions were developed in chloroform methanol water (40 40 10). For separation of gangliosides, chloroform methanol 2.5N aqueous NH4OH (60 40 9) was used. 

We have also found that Thy-1.1 migrates slightly faster than Thy-1.2 in solvent system 1. Structural differences between Thy-1.1 and 1.2 presumably result in different mobility in either solvent system. We found lymphoma cell lines to be a good in vitro source of Thy-1 glycolipid after labeling with either [l-- C]palmitate or [1- C] ManNAc. About 30 compounds were labeled with both plamitate and ManNAc in these cell lines. Additional compounds labeled with palmitate were neutral glycolipids. 

D. salina cells exposed to tracer amounts of 1 C-16 0 or 1 C-18 1 for as little as 2 min. incorporate sizeable amounts of radioactivity into all classes of lipids (1). The radioactive fatty acids are initially concentrated primarily in neutral lipids and phospholipids, but with the passage of time the label is slowly transferred into glycolipids (Fig. 1). This pattern reflects an initial concentration of the radioactive fatty acid in lipids of extrachloroplastidal membranes, such as the endoplasmic reticulum, followed by a somewhat delayed movement of the isotope into the chloroplast, where glycolipid synthesis is confined. 

Experiments of in vivo metabolic labelling showed that unusual neutral and anionic glycolipids were synthesized by carrot cells [10]. The synthetic rate of these glycolipids seemed to depend both on the differentiation stage and on the embryogenic potential of the... 

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