Glycodeoxycholic acid

Fig. 3 Electrophoretic ionic mobilities fj, of propranolol as influenced by BS concentration buffer x mM BS, 20 mM phosphate, pH = 7.4, detection 220 nm m— electrophoretic ionic mobility /r, GC—glycocholic acid, GDC—glycodeoxycholic acid, GCDC—glycochenodeoxycholic acid.

Fig. 4 Electropherogram of cephalosorins (1—cefpim, 2—cefpirom, 3—cepha-loridin, 4—cephlexin, 5—cefaclor, 6—cefuroxim, 7—cefotaxin) in a microemulsion system. Buffer, pH 7.0, 10 mM phosphate containing 6.49% l-butanol/0.82% ra-heptane, 1.44% glycodeoxycholic acid (GDC), and 5.685% Tween. Capillary, 48.5- (40 cm to detector) X 50-/rm ID 30 kV detection, 265 nm. (From Ref. 14.)...

Table 5.4.6 Serum BA composition in healthy subjects (1,2), in a patient (3) with primary biliary cirrhosis (PBC) before (A) and after (B) treatment with ampicillin and in a patient (4) with PBC before (A) and after (B) treatment with UDCA. Results are expressed in nmol/ml. RDS% of three measurements was in the range 3.1-7.5%. - Means -detectable value (adapted from [20]). GCA glycocholic acid, GDCA glycodeoxycholic acid, GCDCA glycochenodeoxycholic acid, GLCA glycolithocholic acid,...

Glycollthocholic acid (GLCA) Glycodeoxycholic acid (GDCA) Glycocholic acid (GCA)... 

Glycodeoxycholic acid recrystallises from H2O or aqueous EtOH with 1 mol of H2O and is dried at 100 in vacuo. Its solubility in EtOH is 5%. [UV Lindstedt Sjovall Acta Chem Scand 11 421 1957] The Na ifl/f recrystallises from EtOH/Et20 with m 245-250° and [a] +41.2° (c 1, H2O) [Wieland 7/oppe Seyler s Z... 

C. cholic acid DC, deoxycholic acid, GC, glycocholic acid GDC, glycodeoxycholic acid. E. lentum [47], C. leptum (52), Clostridium sp. [46],... 

Glycodeoxycholic acid Glycocholic acid Taurocholic acid... 

Mosbach et al, (35) have used cholestyramine to confirm that the la-hydroxylation of cholesterol is the rate-limiting step in bile acid synthesis. Because of the loss of cholesterol from the enterohepatic circulation, there is a marked increase in cholesterol synthesis during administration of cholestyramine to rats (27) or man (36). Mosbach et aL, using perfused rabbit liver, showed that the biliary content of glycocholic acid rose from 0.34 to 3.3 mg, while the content of glycodeoxycholic acid fell from 7.4 to 3.7 mg. The conversion of radioactive acetate, mevalonate, or cholesterol to bile acids was increased from five- to twentyfold, but the conversion rate of 7a-hy-droxycholesterol to cholic acid was unchanged. The formation of 7a-hy-droxycholesterol from cholesterol is enhanced by treatment with cholestyramine (37,38). 

Fig. 1 Densitometric profiles obtained for the investigated bile acids on aluminum plate precoated with silica gel 6OF254 (E. Merck, 1.05554) using n-hexane-ethyl acetate-acetic acid (22 21 5, v/v). Glycocholic acid (GC), glycodeoxycholic acid (GDC), cholic acid (C), glycohthocholic acid (GLC), cheno-deoxycholic acid (CDC), deoxycholic acid (DC), lithocholic acid (LC). (Camag densitometer, wavelength A = 250 nm slit dimensions 6 x 0.2 mm scanning speed 40 nm/sec).

Glycodeoxycholic acid (GDC) 393 (giay/blue) 345 (navy blue)... 

Thirteen free and conjugated bile acids (e.g., cholic, taurocholic, deoxycholic, lithocholic, taurolithocholic, glycodeoxycholic acids) were baseline resolved on a 35 C PLRP-S column (pulsed amperometric detector at +0.03V vs. Ag/AgCl). A 55-min 20/60/30-> 29/51/20 acetonitrile/water/water (0.5 M NaOH) gradient was used [1066]. The presence of 0.1 M hydroxide is essential for the amperometric detection of the hydroxyl fimctional group on the bile acids. However, the authors... 

Fig. 33. Equilibrium pH at point of precipitation of deoxycholic acid (O) and of glycodeoxycholic acid ( ) and ratios of the number of molecules of bile salt necessary to carry one molecule of bile acid in solution (J) in titrations of varying mixtures of...

The approximate Rf values and order of migration should be as follows glycoli-thocholic acid, 0.10 glycodeoxycholic acid, 0.17 glycochenodeoxycholic acid, 0.20 taurodeoxycholic acid, 0.23 taurochenodeoxycholic acid, 0.26 glycocholic acid, 0.31 and taurocholic acid, 0.38. 

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