Glycoconjugates hydrolysis

Because of the numerous methods available for the hydrolysis of various glycoconjugates, including glycoproteins, mucins, glycosaminogly-cans, and gangliosides, it is useful to arrange these methods according to the type of acid employed. Some studies compared a particular method of... 

Honda and coworkers used 4 M hydrochloric acid for 6 h at 100° for the hydrolysis of nondialyzable glycoconjugates when determining amino monosaccharides, but preferred 2 M CF3CO2H for 6 h at 100° when determining the neutral monosaccharides and uronic acids, as these compounds are subject to more-severe degradation by 4 M hydrochloric acid. They obtained complete hydrolysis (with >90% recovery of monosaccharides added prior to hydrolysis) by using these two sets of hydrolytic conditions. 

In the literature, there are several examples of partial hydrolysis of polysaccharides or glycoconjugates containing 2-amino-2-deoxyhex-ose residues. One example is the hydrolysis of carboxyl-reduced, N deacetylated chondroitin, with the formation of 4-0-(2-amino-2-deoxy-/3-D-galactopyranosyl)-D-glucose.51 Another is the isolation of 6-0-(2-amino-2-deoxy-/8-D-galactopyranosyl)-D-galactose after N-de-acetylation and partial hydrolysis of the pentasaccharide (19) obtained... 

Sialidase or NA is the second glycoprotein on the virion surface. One of the most important functions of sialidase is associated with the release of virus progeny from infected host cells to repeat the cycle of infection [6, 50], The enzyme catalyzes the hydrolysis of sialic acid residues from glycoconjugates on the cell surface with either Neu5Ac(a2 3)Gal or Neu5Ac(a2 6)Gal linkages (Gal=galactose) [60, 61]. 

Bik inhibitory strength and selectivity for serine proteases vary greatly with peptide sequence and posttranslational modifications such as fragmentation and glycoconjugation [6, 14, 15]. Predicted Bik fragmentation sites via trypsin binding and/or hydrolysis are shown (Fig. 2). 

Wirth, J, Guo, W., Baumes, R. Gunata, Y. Z. (2002). Volatile compounds released by enzymatic hydrolysis of glycoconjugates of leaves and grape berries from Vitis vinifera Muscat of Alexandria and Shiraz cultivars. J. Agric. Food Chem., 49, 2917-2923. 

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