Glycine residues ribonuclease

Fig. 11. (a) Far ultraviolet rotatory dispersion of ribonuclease. Corrected mean residue specific rotation vs. wavelength [to R = [aLAf/100 [3/(n2 + 2)l where a — specific rotation, M mean residue weight, and n = solvent refractive index. Bars give maximal deviation at peaks. Reproduced from Jirgensons (311). (b) Near ultraviolet rotatory dispersion of 0.48% pancreatic ribonuclease in a 1-mm cell, in (a) 0.15 M phosphate buffer at pH 62 (b) 0.15 M glycine-NaOH buffer at pH 11.5 (c) 0.1 N HC1 (d) 15% sodium dodecyl sulfate. Reproduced from Glazer and Simmons (313). (c) Far ultraviolet circular dichroic spectra of RNase-A, RNase-S, and S-protein at 25° and 3°. Reproduced from Pflumm and Beychok (313). (d) Near ultraviolet circular dichroic spectra of RNase-A as a function of pH. Reproduced from Pflumm and Beychok (313). 

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