Glycerol substrate model

Figure 2.9 Schematic model of the (R)-enantiomer of a secondary glycerol-like substrate as its tetrahedral intermediate inside the active site cleft of hpase B from Candida antarctica (CALB). The large group (-CH2-0-Rj) and the acyl part is inserted hke a V-shape into the cleft while the small group (-CH2-R2) at the stereocentre is located in the stereospecificity pocket which is next to the site of reaction.

TABLE 16.1 Selection of Superior Succinate Producer Organisms, the Corresponding Production Conditions and Important Production Parameters of the Resulting Succinate Production Processes Utilizing Two Important Model Substrates for Implementation in the Concept of Biorefinery—Glucose and Glycerol... 

The evaluation of the data obtained fi om more than 50 substrates permitted the construction of a general model of a substrate that would be accepted by glycerol kinase (Fig. 2.14). 

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