Glycerol-3-phosphate oxidase

An advanced technique for the clean generation of (25) in situ is based on the oxidation of i-glycerol 3-phosphate (35) catalyzed by microbial flavine-dependent glycerol phosphate oxidases (GPO) (Figure 10.19, box) [102]. This method generates... 

The latest, and most advanced, technique uses an inexpensive flavine-dependent glycerol phosphate oxidase (GPO, EC 1.1.3.21), found in several microorganisms (Scheme 13), for air oxidation of L-glycerol 3-phosphate 65 to generate DHAP practically quantitatively and in high chemical purity [200]. A separate cofactor regeneration step has become obsolete because the reduced... 

The application of the biocatalysts in this overview is limited to these stable enzymes, which do not need cofactors, such as the various hydrolytic enzymes, some lyases, transferases and isomerases. In addition to these groups, oxidoreductases, which demand NAD or NADP as cofactors, some pyridoxyl-phosphate dependent lyases with simple systems for cofactor regeration and finally, various aldolases in combination with L-glycerol- phosphate oxidase and catalase are useable to some extent in cell-free form. 

A combination of glycerol phosphate oxidase (GPO), catalase, and L-rhamnulose-1-phosphate aldolase (RhuA) allowed for an efficient conversion of aminoglycerol into 1-deoxy-l-phosphoramido-L-fructose (Scheme 24). 

Reagents i, Glycerol phosphate oxidase ii, galactose oxidase iii, L-rhamnulose phosphate aldolase iv, phosphatase... 

Dihydroxyacetone phosphate (41) was prepared enzymatically from glycerol 1-phosphate (74) by the action of glycerol phosphate oxidase [177]. Analytical thin-layer chromatography was performed on silica gel plates using a 1 1 mixture of sat. ammonia-ethanol for development, and anisaldehyde stain for detection. The aldolases are commercially available or can be purified in accordance with published procedures [150]. Activity of aldolases (1 unit catalyzes cleavage of 1 pmol L-ketose 1-phosphates (43/45) per minute at 25 °C [150]) and amounts of 41 were determined photometrically by an assay coupled with glycerol phosphate dehydrogenase-catalyzed NADH oxidation [149]. 

L-glycerol-3-phosphate oxidase, l-GPO (EC 1.1.3.21, from Thermophilus bacillus) (45 units)... 

Glycerol-3-phosphate oxidase (GPO), triglyceride determination, 633 GlycerophosphochoUne, unsaturated, 737 GlycerophosphoUpid hydroperoxides, human plasma, 690... 

GOX (glucose oxidase), 631, 636, 637 GPC (unsaturated glycerophosphocholine), 737 GPO (l-glycerol-3-phosphate oxidase), 633 Graft polymers cellulose, 698 cross-linking initiators, 706 ozonized polymers, 622 Griesbaum co-ozonolysis, 1,2,4-tiioxane antimalarials, 1331... 

Compagnone et al. [5] Glycerol Alcoholic fermentation Glycerokinase (GK) and glycerol-3-phosphate oxidase (GPO)/ covalently immobilised (Bioreactor of GK and bioelectrode of GPO) Pt-based hydrogen peroxide probe/+650mV vs. Ag/AgCl -... 

Immobilisation of glycerokinase in a glass beads reactor coupled with glycerol-3-phosphate oxidase on a preactivated Immobilon AV membrane kept at the electrode surface. 

Fiaure 27. Synthesis of a ferrocene-modified glycerol-3-phosphate oxidase via a PEG spacer and a cyanuric chloride anchor group (n = 5-19) (activity of modified GPO 37% of the unmodified enzyme). 

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