Glycerol phosphate isomer

Oxidation by raising the pH (to 7.5) phytase activity is switched off hydrolysis is prevented. Oxidation of L-glycerol-3-phosphate to DHAP by GPO at 55% glycerol (v/v) is quantitative. Catalase is added to suppress the build-up of hydrogen peroxide. The D-isomer is converted back into glycerol and phosphate in the last step. 

Condensation of (441) [306, 307] with 1,2-di-O-stearoyl-DL-glycerol 3-phosphate in the presence of 2,4,6-triisopropylbenzene sulfonyl chloride in pyridine gave a mixture of the required products (442) (52%) and the isomer (443) (26%) which were separated by chromatography. The anilino groups were removed from (442) by the... 

Figure 1. Schematic representation of the brain inositol signaling system. The quantities of IMPase isoenzymes and IPPase are increased by chronic lithium treatment occurring at either the gene or protein levels. Inositol in this diagram indicates the myo-inositol isomer. Calbindin -calcium binding protein DAG- diacyl glycerol Gq-GTP binding protein IMPase 1 — inositol mono phosphatase 1 IPPase- inositol polyphosphate 1-phosphatase Ins(l)P, Ins(3)P, Ins(4)P-inos-itol monophosphates Ins(l,3)P2 - inositol 1,3-bisphosphate Ins( 1,4)/ 2 - inositol 1,4-bisphos-phate Ins(3,4)/)2- inositol 3,4-bisphosphate Ins (1,4,5)P3 - inositol 1,4,5-trisphosphate Ins( 1,3,4)/ 3 - inositol 1,3,4-trisphosphate Li+-lithium PA - phosphatidic acid PI- phosphatidyl inositol PIP- phosphatidyl inositol 4-phosphate PIP2- phosphatidyl inositol 4,5-bisphosphate PIP3- phosphatidyl inositol 3,4,5 trisphosphate PLC - phospholipase-C, VPA-valproate.

Glycerol formed by lipolysis is absorbed by the liver and phosphor) lated. It is then oxidized to dihydroxyacctone phosphate, which is isomer ized to glyceraldehyde 3-phosphate, This molecule is an intermediate in both the glycolytic and the gluconeogenic pathways. 

Fig. 8. Outlines of the method to identify isomers of phosphoribosyl pentapeptide. 1-, 2-, and 3-peptidyl ribose 5-phosphate are expected to give, after oxidation by NaI04 (pH 7, room temperature, 60 min), reduction by NaBH4, and digestion with alkaline phosphomonoesterase, glycerol (C3), ribitol (C5), and erythritol (C4), respectively. For reference, the expected production of ethylene glycol (Cj), which is mostly distilled out at the step of evaporation in vacuo, from free ribose 5-phosphate is also shown...

The analysis of DL-glycerol-3-phosphate disodium hexahydrate (33) was carried out to provide geometrical details for correlation with structural studies of enzymes, the D-isomer being an inhibitor of triose phosphate isomerase (TIM), whereas the L-isomer is both a substrate and inhibitor for glycerol kinase. The most intmesting feature of the molecular geometry is... 

Monoacylglycerols and diacylglycerok can ako be esterified using phosphorus pentoxide. For example, esterification of 1,2-diacyl-sn-glycerol yields phosphatidic (1,2-diacyl-sn-glycerol 3-phosphate) and the corresponding bisphosphatidic acid and their positional isomers. Both acids are natural-identical products, which serve as substitutes for lecithin. Ammonium phosphatides (E442) are approved in the EU as emulsifiers. 

Dbrmann P, Frentzen M, Ohlrogge JB. Specificities of the acyl-acyl carrier protein (ACP) thioesterase and glycerol-3-phosphate actyltransferase for octadecenoyl-ACP isomers. Plant Physiol 1994 104 839-844. 

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