Glycan fluorescent labeling

Bigge, J.C., Patel, T.P., Bruce, J.A., Goulding, P.N., Charles, S.M., and Parekh, R.B. (1995) Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal. Biochem. 230, 229-238. 

Zhuang et al. [152] derivatized glycan samples with 8-aminopyrene-l,3,6-trisulfonic acid to get a charge for electrophoresis and a fluorescent label for detection. They analyzed glycan derivatives in the blood of cancer patients... 

The general form for oligosaccharide profiling is release of the glycans, derivatization (e. g. fluorescent labeling), then analysis by HPLC, CE, or MS. 

C) Separation of glycans Chromatography 1. Normal phase HPLC Capable of resolving subpicomolar quantities of mixtures of fluorescently labelled neutral and acidic N- and O-glycans simultaneously [265]... 

Figure 35 Glycan photocross-linking probes for fluorescent labeling. Photocross-linkers are shown in red and fluorophores are in blue, (a and b) Lactose molecules modified with a benzophenone cross-linker and a functional group that can react through click chemistry. After photocross-linking, the adducts are reacted with an azide or alkyne-functionalized fluorophore to fluorescently label the protein-binding partner, (c) Multivalent mannose or fucose (represented in green) probes bear a benzophenone cross-linker and a Cy3 or Cy5 fluorescent label.

Key words Glycan, Microarray, Lectins, Glycan-binding proteins. Fluorescent labeling... 

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