Glycan biosynthesis inhibitors

Fig. 11.1-11 Tunicamycin 10 is an inhibitor of N-glycan biosynthesis, while natural products such as 1-deoxymannojirimycin inhibit glycosidase function.

In transfected mammalian cells inhibition of N-glycan addition by treatment with tunicamycin prevented secretion of factor VIII, whereas treatment with an inhibitor of complex oligosaccharide biosynthesis, deoxymannojirimycin, did not affect secretion, although the specific activity of factor VIII was slightly increased [45]. Thus, the presence of complex oligosaccharide was not required for secretion or functional activity of factor VIII. A23187 treatment inhibited addition of serine/threonine (O)-linked glycans to factor VIII. 

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