Glutaraldehyde concentration used

B. Crosslinking the protein in TBSV with glutaraldehyde diminished the absorption. (BMV, at the particle concentration used for these experiments, could not be crosslinked). 

At 0.2 0 glutaraldehyde concentration there is a relatively law probability of cross-linking and hence a relatively low number of active cross-link sites. Therefore, a plateau of UTS is reached rather than a peak since the active sites in the surface region are used up and cross-linking ceases. Cross-linking has therefore been stopped before the surface imperfections have become an lified as stress concentrations. This allows fibers to be cross-linked for the "optimum time which heretofore was impossible due to the manual nature of the fiber production procedure. 

In a second example, a cell—gelatin mixture is cross-linked with glutaraldehyde (43). When soluble enzyme is used for binding, the enzyme is first released from the cell, then recovered and concentrated. Examples of this type of immobilization include binding enzyme to a DEAE-ceUulose—titanium dioxide—polystyrene carrier (44) or absorbing enzyme onto alumina followed by cross-linking with glutaraldehyde (45,46). 

Catalase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated Teflon membrane served as enzyme electrode to determine hydrogen peroxide [248], The electrode response reached a maximum when 50mM phosphate buffer was used at pH 7.0 and at 35°C. Catalase enzyme electrode response depends linearly on hydrogen peroxide concentration between 1.0 X 10-5 and 3.0 X 10-3 M with response time 30 s. 

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