Glutamate dehydrogenase bovine

Glutamate dehydrogenase (bovine) -40 -8 300 -1 000 000 Enzyme interconverts glutamate and a-ketoglutarate and NH4+... 

Glutamate dehydrogenase Bovine liver Association 7.8 25 21 Heremans (1974)... 

EC1.4.1.3 glutamate dehydrogenase, bovine NAD(P) -dependent (amino acid dehydrogenase)... 

BSA monomer, ovalbumin (chicken), P-lactoglobulin (bovine milk), serum albumin (human), carbonic anhydrase (bovine), L-glutamic dehydrogenase (bovine liver), a-chymotrypsin (bovine), a-chymotrypsinogen A (bovine), immunoglobulin (bovine milk), pepsin, trypsin (bovine), and heparin were from Sigma. RNase and lysozyme (egg white) were from Calbiochem. The recombinant human basic fibroblast... 

Bovine Liver Glutamate Dehydrogenase Henryk Eisenberg, Robert Josephs, and Emil Reisler... 

Chen S-S, Engel PC (1975) The equilibrium position of the reaction of bovine fiver glutamate dehydrogenase with pyridoxal 5 -phosphate. Biochem J 147 351-358... 

Figure 2. SDS gel electrophoresis of the products of partial cystine cleavage for several test proteins. A. molecular weight standards, B. yeast alcohol dehydrogenase. C. P-lactoglobulin, D. hen egg lysozyme, E. ovalbumin, F. calf fetal serum fetuin. Molecular weight standards are indicated by arrows on the left side of the gel and are bovine serum albumin (66,300), bovine liver glutamate dehydrogenase (55,400), porcine muscle lactate ddiydiogenase (36,500), bovine erythrocyte carbonic anhydrase (31,000), soybean trypsin inhibitor (21,500), hen egg lysozyme (14,400), bovine lung aprotinin (6,000), unresolved bovine pancreatic insulin A and B chains.

Figure I. Influence of storage (+4°C) on the activity of glutamic dehydrogenase in muscle press juice from bovine longissimus dorsi (time...

L-Glutamate Dehydrogenase Origin bovine liver Roche Diagnostics L-Glutamate Dehydrogenase (G1DH), lyo. 

Substrate inhibition of bovine liver glutamate dehydrogenase has not been studied in such detail. It is most marked when the NAD(P) concentration is also large (11), and is relieved by ADP. This appears to be the reason why ADP activates the enzyme when large glutamate and... 

Fia. 4. Amino acid sequences of bovine (B), chicken (C), and rat (R) liver glutamate dehydrogenases. The chicken enzyme possesses three additional residues at the NHa-terminal end with the NHa-terminal residue indicated as cysteine although it was found as cysteic acid. The unpublished data (260) for the rat enzyme were... 

The reactive residues of glutamate dehydrogenase have been probed with a variety of reagents and methods. Unless noted otherwise, the studies have been conducted with the bovine enzyme. 

In many cases, 5 -FSBA reacts at active sites however, in bovine liver glutamate dehydrogenase, all the fluorosulfonylbenzoyl nucleosides react at regulatory sites. This enzyme is a hexamer of identical subunits, each of which has two regulatory sites for ADP, two for GTP, and two for NADH (one catalytic... 

Other proteins similarly studied are bovine serum albumin 5, 6, 36, 65, 67) and ovalbumin 65) actin 61) fumarase 43) a-chymotrypsin 44) lysozyme 59, 64) arachin 5) 0-lactoglobulin and /c-casein 45) 7-globulin 56) ribonuclease 24, 35, 68) glutamic dehydrogenase (7) soybean trypsin inhibitor 15, 57) and pepsinogen 14, 21, 58). 

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