Glucosides emulsin effect

Fischer was intrigued by the fact that emulsin caused hydrolysis of both / -glucosides and / -galactosides but had no effect on either the a- or / -xylo-sides (33). Since, at the time, Fischer expected glycosides to be furanosides, he suggested that both the enzymes required the presence of a free hydroxyl group at position 5 of a hexoside. 

Emil Fischer developed a strong interest in the structural requirements for enzyme activity as the result of effects of changes in the structures of the a-and -methyl glucosides on their properties as substrates for the enzymes invertin and emulsin, which, as we have seen, he had shown to be a- and yS-glucosidases, respectively. As already mentioned, he was fascinated in 1895 by the fact that emulsin had no effect on either the a- or /2-methyl xylosides (33). In a 1912 publication with Karl Zach (34), he reported that -methyl 6-deoxyglucoside was hydrolyzed by emulsin and wrote ... 

Going to the next more complex situation, that of enzymic reactions, it can be shown that the linear combination of ir and a can account for the substituent effects in the hydrolysis of phenyl glucosides by emulsin . Using regression analysis, eq 10-13 are derived to illustrate how one can... 

Nonetheless, values for aryl glycosides hydrolysed by glycosidases continue to be measured the first such, for aryl p-glucosides hydrolysed by almond emulsin (which measured the effect of leaving group acidity from tabulated Hammett a constants, rather than directly from the leaving group pK ) dates... 

---