Glucose standard solution

The gel disks used to measure glucose contained 40% (w/w) DMAEM and 3.3% (w/w) N,N-methylenebisacrylamide. The gel was placed in aqueous glucose standard solutions, and the change in diameter was measured as above. 

Glucose Standard Solution Transfer about 300 mg, accurately weighed, of USP Dextrose Reference Standard, previously dried in vacuum at 70° for 4 h, into a 1000-mL volumetric flask, dissolve in and dilute to volume with water, and mix. Allow to stand for 2 h to allow mutarotation to occur, then transfer 20.0 mL to a 100-mL volumetric flask, dilute to volume with water, and mix. Prepare fresh on the day of use. 

Glucose Standard Solutions Transfer 100 mg of a-D-glu-cose (Aldrich, or equivalent), accurately weighed, into a 500-mL volumetric flask, and dilute to volume with water. Dilute 5 aliquots of the solution with water to obtain the following concentrations of standard 50, 40, 20, 10, and 5 (ig/mL. Phenol Solution Add 20 mL of water to 80 g of phenol. Sample Solution Transfer approximately 250 mg of sample, accurately weighed, into a 250-mL volumetric flask, and dilute to volume with water. 

Standard Curve On a daily basis, pipet 2.0 mL of each Glucose Standard Solution into separate, acetone-free, 15-mL screw-cap vials. Add 0.12 mL of the Phenol Solution, and mix gently. Uncap each vial and rapidly add 5.0 mL of sulfuric acid. Immediately recap each vial, and shake vigorously. 

Glucose Standard Solution Dissolve 36.0 mg of anhydrous dextrose in Phosphate Buffer in a 1000-mL volumetric flask, dilute to volume with water, and mix. 

After equilibration, add 1 mL of Phosphate Buffer to tube 1 (substrate blank), 1 mL of Glucose Standard Solution to tube 2 (glucose standard), 4 mL of Neocuproine Solution A and 1 mL of the Test Preparation to tube 3 (enzyme blank), and 1 mL of the Test Preparation to tube 4 (sample). Prepare a fifth tube for the buffer blank, and add 3 mL of Phosphate Buffer. 

D-Glucose standard solutions in water 3.00 mM, 11.10 mM, and 16.65 mM Lactate assay reagent consisting of ... 

Subtract the absorbance value of G1 (blank) from the rest of the samples. Construct a standard curve of A340 versus micromoles of glucose for your three glucose standard solutions (G3, G4, and G5). 

Small (13 X 100 mm) glass test tubes—1000 required. Spectrophotometer with cuvettes—At least 1 required. Glucose standard stock solution (44.48 mM) —Dissolve 80.1 mg of D-glucose in 10 ml of distilled water. 3.00 mM glucose standard solution —Add 67 pi of... 

Fig. 12.3. In vivo experiment with 10-mm hollow fibre with 20,000 MWCO inserted subcutaneously in a rat and a wall-jet cell as detector. The peaks correspond to spikes of glucose standard solution sent directly into the fiow cell by the injection valve, while the continuous line represents the subcutaneous glucose monitoring through the microdialysis probe.

Fig. 47. Comparison of stationary and kinetic response curves of the measurement of glucose standard solution and serum. 1 standard solution, 5.5 mmol/1 2 control serum Serulat (VEB Sachsisches Serumwerk Dresden, GDR), 4.8 mmol/1.

Response of a glucoamylase — GOD electrode to glucose standard solution (11.11 mM, —) and to control serum containing 11.13 mM glucose and 0.3 U/mL of a-amylase ( ). Reproduced from [377] with permission from Marcel Dekker, Inc. 

Different concentration glucose standard solutions (1 mL) is pipetted into the test tube with plug. 1 mL distilled water, 1 mL phenol solution (8%) and 5 mL of sulfuric acid were added respectively, fiiUy mixed, allowed to stand for 10 min and vibrated. Then it was put in water (25°C to 30°C) for 20 min. The solutions shows gradually deepened orange color according to the concentration from low to high. Absorbency value was determined and standard curves were drawn. 

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