Glucose sensor Michaelis-Menten constants

The linear response range of the glucose sensors can be estimated from a Michaelis-Menten analysis of the glucose calibration curves. The apparent Michaelis-Menten constant KMapp can be determined from the electrochemical Eadie-Hofstee form of the Michaelis-Menten equation, i = i - KMapp(i/C), where i is the steady-state current, i is the maximum current, and C is the glucose concentration. A plot of i versus i/C (an electrochemical Eadie-Hofstee plot) produces a straight line, and provides both KMapp (-slope) and i (y-intercept). The apparent Michaelis-Menten constant characterizes the enzyme electrode, not the enzyme itself. It provides a measure of the substrate concentration range over which the electrode response is approximately linear. A summary of the KMapp values obtained from this analysis is shown in Table I. 

The linear respcmse range of the sensors can be estimated from a Michaelis-Menten analysis of the glucose calibration gra in Figures 4 and 5. The apparent Michaelis-Menten constant Km PP can be determined from the electrochemical Eadie-Hofstee form of the Michaelis-Menten equation (30 ) ... 

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