Glucose determination with enzyme thermistors

Quantitative determinations of L-ascorbic acid, D-galactose, D-glucose, cellobiose, lactose and sucrose were made with "enzyme-thermistors", in which the heat produced by reaction of a sugar with an immobilized enzyme (e.g , Invertase for sucrose) in a small column is monitored by a thermistor placed in the column eluent the procedure is suitable for discrete samples or continuous monitoring. ... 

Kiba N, Tomiyasu T and Furusawa M 1984 Flow enthalpimetric determination of glucose based on oxidation of 1,4-benzoquinone with use of immobilized glucose oxidase column Talanta 31 131-2 Scheller F, Siegbahn N, Danielsson B and Mosbach K 1985 High-sensitivity enzyme thermistor assay of L-lactate by substrate recycling Anal. Chem. 57 1740-3... 

Test strips, which are available for the determination of about ten low-molecular mass substances (metabolites, drugs, and electrolytes) and eight enzymes [356], can be considered as precursors of optoelectronic biosensors. Efficient optoelectronic sensors based on immobilized dyes have been devised for the determination of glucose, urea, penicillin, and human serum albumin [357]. Other approaches use immobilized luciferase or horseradish peroxidase to assay ATP or NADH or, when coupled with oxidases, to measure uric acid or cholesterol. These principles have not yet been generally accepted for use in routine analysis. Thermistor devices involving immobilized enzymes or antibodies for a number of clinically relevant substances have also been described. Thermometric enzyme linked immunosorbent assays are being routinely employed for monitoring the production of monoclonal antibodies. 

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