Gluconolactone hydrolase

A. glucose-6-phosphate dehydrogenase, NADH, gluconolactone hydrolase, 6-phosphogluconate dehydrogenase, ribulose-5-phosphate epimerase... 

Thus a biospecific adsorbant was obtained selective desorption should therefore permit successive elution of cellulolytic enzymes. It was thus found that the method was very useful in the purification of the cellobio-hydrolases from Tr. r., starting from very crude culture filtrates. Addition of glucose (0.1M) or gluconolactone suppressed the action of glucosidases present, preventing deterioration of the columns. The capacities exceeded 10 mg CBH I per ml gel (prepared with CNBr activated Sepharose). 

Stachybotrys atra (235). An intracellular enzyme from this fungus, which released glucose as the sole product from / -l,4-oligoglucosides up to CL 11 and was not inhibited by 1,5-gluconolactone (236), is apparently an exo-hydrolase. The / -l,4-glucosyl linkage in p-nitrophenyl-a-cellobioside was also hydrolyzed. 

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