GFP fluorescence

Leiderman P, Huppert D, Agmon N (2006) Transition in the temperature-dependence of GFP fluorescence From proton wires to proton exit. Biophys J 90 1009-1018... 

Finally, a nonfluorescent YFP variant has been constructed for use as a FRET acceptor for GFP [89]. This allows the detection of the complete emission spectrum of GFP, while the FRET efficiency is high (R0 = 5.9 nm) due to strong overlap of the GFP fluorescence and YFP absorbance band. The occurrence of FRET was detected by a reduction in the excited state lifetime of the GFP by FLIM. The main disadvantage is that the presence of the acceptor cannot be detected in living cells. 

Fig. 11.2 Localization of GFP and calsequestrin (CSQ) in neonatal rat cardiac myocytes. Myo cytes were infected with a recombinant adenovirus containing the cDNAs of GFP and CSQ. Both cDNAs were expressed under control of a separate cytomegalovirus promoter. Expression of CSQ and the coexpressed GFP was detected by fluorescence microscopy. Left-. GFP fluorescence (green), middle immunostaining of CSQ (red), right overlay of GFP fluorescence and immunos taining. Nuclei were counterstained with DAPI (blue). Courtesy of Ulrich Gergs...

Figure 5.7 Detection of PNA binding-mediated GFP gene expression in CV1 cells. Twenty hours post-injection of plasmid or plasmid-PNA complexes, GFP expression was determined. GFP fluorescence is shown in the top panels and the rhodamine-labeled BSA signal is shown in the bottom panels. (A) and (D), CV1 cells injected with pUSAG3.PNA-l (B) and (E), CV1 cells injected withpUSAG3.PNA-2 (C) and (F), CV1 cells injected with pUSAG3 plasmid DNA alone. In all cases, rhodamine-labeled BSA was co-injected with the DNAs to the cells, (see Color Plate 4)...

Fig. 15 Internalization and gene expression of pDNA released from a multilayer assembly. Release of pDNA occurs upon degradation of T4 polyamide. Notable increase in fluorescence intensity overtime is observed in the flow histograms. Gene expression (measured by intracellular GFP fluorescence) does not increase at the same rate as DNA uptake. Figure adapted with permission from [151]. 2009 Elsevier...

Fig. 8.1. rAAV2/l and rAAV2/5 transduce the HPC more efficiently than rAAV2/2. (A-C), Green shows GFP fluorescence red is NeuN immunoreac-tivity, which labels the neuronal cell bodies in the hippocampal formation. Yellow represents the merge of neuron-specific staining (red) and GFP (green)... 

Fig. 2. Representative HUVEC transfection efficacy of C32 and method for FACS gating. The one-dimensional histogram of C32/DNA transfected cells (56.5% positive) (left) includes some falsely positive cells that are excluded during the two-dimensional analysis of the same data set as shown by the FACS density plot gating for the negative control (0% positive) (middle) and the C32/DNA transfection (44.7% positive) (right). Ratio of GFP fluorescence (x-axis) to background fluorescence (y-axis) is used to accurately gate positive cells.

Fluorescent cholera toxin Fluorescent dextran conjugates Fluorescent Dil-LDL Fluorescent fusion proteins (GFP) Fluorescent / neutralizing IgG Fluorescent transferrin Hypertonic sucrose Ikarugamycin... 

Fig. 7.4. GFP PAR-2 (a, c) and NMY-2 GFP (b, d) on the cortex. The scan path of sFCS measurements is indicated by a white circle, (c) and (d) indicate the non-uniform fluorescence pattern of GFP PAR-2 and NMY-2 GFP. Fluorescence intensity is plotted as a 2D-plot, where one horizontal line represents one full circular scan with each subsequent cycle displayed on the vertical axis from top to bottom. Scale bar represents 10 xm. a anterior, p posterior...

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