Germination time sequence

The subcellular localization of the starch biosynthetic and degradative enzymes of spinach leaves was carried out by measuring the distribution of the enzymes in a crude chloroplast pellet and in separated components of a protoplast lysate. The enzymes which were involved in the syntheses of starch were detected in the chloroplasts, whereas some of the enzymes involved in the degradation of starch were mainly in the soluble protein fraction but were also found in the chloroplast. The digestion pattern of the amylase on amylopectin as substrate indicated that the enzyme was an a-amylase from its endo-lytic activity, but displayed properties unlike the typical a-amylase isolated from endosperm tissue. A time-sequence analysis of the starch digestion pattern in germinating... 

This table is an attempt to place in a chronological sequence the events leading up to carbohydrate breakdown. It should be noted that this scheme only applies to work done on Proctor barley, imbibed and germinated under non-malting conditions. The times should be taken as being very approximate since the literature drawn upon to devise this scheme is often equivocal. Related events for which the published chronology is very inconsistent are marked with asterisks... 

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