Genetic engineering polymer synthesis

While biological synthesis of polymers confers many advantages, it also imposes some limitations. As described previously, there are several issues that must be considered when designing the monomer and concatamer gene sequences. Furthermore, the potential toxicity of the genetically engineered polymer to the expression host, and the resultant effect on the integrity of the product must be considered. 

Biomaterials, Synthesis, Fabrication, and Applications Bioreactors Hybridomas, Genetic Engineering of Mammalian Cell Culture Metabolic Engineering Polymers, Synthesis Surface Chemistry... 

Yu SM, Soto CM, Tirrell DA (2000) Nanometer-scale smectic ordering of genetically engineered rodlike polymers synthesis and characterization of monodisperse derivatives of poly(y-benzyl a, L-glutamate). J Am Chem Soc 122 6552-6559... 

Most modern methods (for DNA or RNA) are essentially combinations of improved chemical and biochemical techniques of this kind. Polymers containing over 150 nucleotide units have been made, and their laboratory synthesis has now become a subject of great importance in connection with the preparation of artificial genes for use in genetic engineering. Genes can be effectively copied by PCR (Chapter 11.6). 

Rodriguez-Cabello JC, Girotti A, Ribeiro A, Arias FJ (2010) Synthesis of genetically engineered protein polymers (recombinamers) as an example of advanced self-assembled smart materials. In Methods in molecular biology (in press)... 

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