Genetic analysis, oligonucleotide probes

Saiki RK, Walsh PS, Levbnson CH., Erlich HA (1989) Genetic analysis of amplified DNA with immobilized sequence specific oligonucleotide probes. Proc. Natl Acad Sd USA 86 6230-6234. 

E. Kawasaki, R. Saiki, H. Erlich, Genetic Analysis Using Polymerase Chain Reaction-amplified DNA and Immobilized Oligonucleotide Probes Reverse Dot-Blot Typing , Meth. Enzymol., 218, 369-381 (1993). 

DNA applications, in particular DNA sequencing, human identification, and genetic analysis dominate the field. Other DNA applications including oligonucleotides, antisense DNA, restriction fragments, plasmids, PCR products, hydridization (DNA probe), and RNA have also been demonstrated [7,8]. 

CIO. Cate, R. L., Ehrenfels, C. W., Wysk, M., Tizard, R., Voyta, J. C., Murphy, O. J., and Bronstein, I., Genomic southern analysis with alkaline phosphatase-conjugated oligonucleotide probes and the chemiluminescent substrate AMPPD. Genet. Anal. Tech. Appl. 8, 102-106 (1991). 

Molecular genetics assays can be designed to amplify both normal and mutant alleles, then to determine which allele is present in a postamplification phase of the analysis. For detection of single base changes, a generic amplification is conducted with primers that flank the site of the mutation. Following PCR, oligonucleotide probes for wild type or mutant are... 

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