Genes protease, transfection

I. Transfection of Genes Encoding Proteases Enhances Metastasis. Transfection of different cell types with the genes for either uPA or CD confers or enhances the metastatic ability of the recipient cells. This has been shown for uPA in mouse L cells, ras-transformed NIH 3T3 cells, and murine B16 mela-... 

High throughput production of recombinant proteins can be achieved by removing the virus screening step and enhancing the rate of transfection by using linearized baculovirus DNA (3). Knock-down of the cysteine protease gene from the baculovirus... 

Unlike the situation in trypanosomes, RNAi does not seem to be operative in Leishmania. However, protein expression can be reduced by antisense RNA. This is usually tudiieved by transfection of parasites with an appropriate gene construct. Antisense RNA has been used to reduce the expression of the membrane protease gp63 and the amastigote-specific protein A2. Loss ofeither of the two proteins was shown to reduce parasite virulence, making them attractive drug targets. 

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